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Stability and Performance of Xanthobacter autotrophicus GJ10 during 1,2-Dichloroethane Biodegradation

机译：1,2-二氯乙烷生物降解过程中自养黄萎菌GJ10的稳定性和性能

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A nucleic acid-based approach was used to investigate the dynamics of a microbial community dominated by Xanthobacter autotrophicus GJ10 in the degradation of synthetic wastewater containing 1,2-dichloroethane (DCE). This study was performed over a 140-day period in a nonsterile continuous stirred-tank bioreactor (CSTB) subjected to different operational regimens: nutrient-limiting conditions, baseline operation, and the introduction of glucose as a cosubstrate. The microbial community was analyzed by a combination of fluorescence in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE). Under nutrient-limiting conditions, DCE degradation was restricted, but this did not affect the dominance of strain GJ10, determined by FISH to comprise 85% of the active population. During baseline operation, DCE degradation improved significantly to over 99.5% and then remained constant throughout the subsequent experimental period. DGGE profiles revealed a stable, complex community, while FISH indicated that strain GJ10 remained the dominant species. During the addition of glucose as a cosubstrate, DGGE profiles showed a proliferation of other species in the CSTB. The percentage of strain GJ10 dropped to 8% of the active population in just 5 days, although this did not affect the DCE biodegradation performance. The return to baseline conditions was accompanied by the reestablishment of strain GJ10 as the dominant species, suggesting that this system responds robustly to external perturbations, both at the functional biodegradation level and at the individual strain level.
机译：一种基于核酸的方法被用来研究由自生黄腐杆菌（Xanthobacter autotrophicus GJ10）主导的微生物群落在降解含1,2-二氯乙烷（DCE）的合成废水中的动力学。这项研究是在140天的非无菌连续搅拌釜式生物反应器（CSTB）中进行的，该反应器采用不同的操作方案：营养限制条件，基线操作和引入葡萄糖作为共底物。通过荧光原位杂交（FISH）和变性梯度凝胶电泳（DGGE）的组合分析了微生物群落。在营养限制条件下，DCE的降解受到限制，但是这并不影响菌株GJ10的优势，FISH确定其占活性种群的85％。在基准运行期间，DCE的降解显着提高到99.5％以上，然后在随后的整个实验期间保持恒定。 DGGE谱显示稳定，复杂的群落，而FISH表明菌株GJ10仍然是优势种。在添加葡萄糖作为共底物期间，DGGE谱显示CSTB中其他物种的增殖。尽管这并没有影响DCE的生物降解性能，但GJ10菌株的百分数在短短5天内下降到了活跃人群的8％。回到基线状态伴随着菌株GJ10作为主要物种的重新建立，这表明该系统在功能性生物降解水平和单个菌株水平均对外部扰动具有较强的响应能力。

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